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Table 1 Primer design for genes analyzed by real-time PCR

From: The role of expression imbalance between adipose synthesis and storage mediated by PPAR-γ/FSP27 in the formation of insulin resistance in catch up growth

SYBR Green RT-PCR Primers

Forward Primer

Reverse primer

Annealing temperature

PPAR-γ

TGTCGGTTTCAGAAGTGCCTTG

TTCAGCTGGTCGATATCACTGGAG

60 °C

FSP-27

AAGGCATCATGGCCCACAG

TCTCCACGATTGTGCCATCTTC

58 °C

SIRT-1

TCCTCACTAATGGCTTTCATTCCTG

GTGCCAATCATGAGATGTTGCTG

57 °C

β-Actin

GGAGATTACTGCCCTGGCTCCTA

GACTCATCGTACTCCTGCTTGCTG

62 °C

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Lipids in Health and Disease

ISSN: 1476-511X