Fig. 2From: Reconstituted high-density lipoproteins promote wound repair and blood flow recovery in response to ischemia in aged miceThe effect of rHDL on wound neovascularization, wound macrophage content and collagen deposition. Two full thickness wounds were created on the back flanks of 24-month-old C57Bl/6 J mice (n = 9/group). Mice received daily topical applications of rHDL (50 μg/wound) or PBS (vehicle). On Day 10 post-wounding, wound tissue was collected and sectioned for: a Capillaries using immunohistochemistry for CD31+ (brown, denoted by arrows); b Macrophage infiltration was identified in wound sections using immunohistochemistry for Mac-3+ (brown, denoted by arrows); c Collagen deposition was determined using Milligan’s trichrome stain (blue). Photomicrographs represent wounds stained for CD31+, Mac-3+ and collagen of PBS and rHDL wounds following sacrifice. Data is represented as mean ± SEMBack to article page