Fig. 3From: Reconstituted high-density lipoproteins promote wound repair and blood flow recovery in response to ischemia in aged micerHDL had no effect on gene expression of angiogenesis markers in wounds. Two full thickness wounds were created on the back flanks of 24-month-old C57Bl/6 J mice (n = 9/group). Mice received daily topical applications of rHDL (50 μg/wound) or PBS (vehicle). On Day 10 post-wounding, wound tissue was collected and RNA was isolated for qRT-PCR to assess gene expression or wound tissue was homogenized and used in ELISAs to assess protein. Measured markers include: a VEGF mRNA; b VEGF protein; c HIF-1α mRNA; d Siah1 mRNA; e Siah2 mRNA; f PHD2 mRNA; and g PHD3 mRNA. Data is represented as mean ± SEMBack to article page