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Fig. 9 | Lipids in Health and Disease

Fig. 9

From: Reconstituted high-density lipoproteins promote wound repair and blood flow recovery in response to ischemia in aged mice

Fig. 9

rHDL infusions increase plasma total cholesterol and HDL cholesterol. Femoral artery ligation was performed on 24-month-old C57B1/6 J mice. Mice received i.v. injections of PBS or rHDL (40 mg/kg) on alternate days following ligation until sacrifice 21 days later. Whole blood was collected via cardiac puncture at the 21 day time point and plasma isolated. a Total cholesterol concentrations were determined enzymatically on mouse plasma using commercially available kits; b HDL cholesterol concentrations were determined by enzymatic assay following precipitation of apolipoprotein B containing proteins with polyethylene glycol; c LDL levels were determined by subtracting total HDL from total cholesterol. d Triglyceride levels were determined enzymatically on mouse plasma using commercially available kits. Data is represented as mean ± SEM. Statistical analysis was performed by unpaired two-tailed t-test. **p < 0.01 vs. PBS control

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