Fig. 9From: Reconstituted high-density lipoproteins promote wound repair and blood flow recovery in response to ischemia in aged micerHDL infusions increase plasma total cholesterol and HDL cholesterol. Femoral artery ligation was performed on 24-month-old C57B1/6 J mice. Mice received i.v. injections of PBS or rHDL (40 mg/kg) on alternate days following ligation until sacrifice 21 days later. Whole blood was collected via cardiac puncture at the 21 day time point and plasma isolated. a Total cholesterol concentrations were determined enzymatically on mouse plasma using commercially available kits; b HDL cholesterol concentrations were determined by enzymatic assay following precipitation of apolipoprotein B containing proteins with polyethylene glycol; c LDL levels were determined by subtracting total HDL from total cholesterol. d Triglyceride levels were determined enzymatically on mouse plasma using commercially available kits. Data is represented as mean ± SEM. Statistical analysis was performed by unpaired two-tailed t-test. **p < 0.01 vs. PBS controlBack to article page