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Fig. 4 | Lipids in Health and Disease

Fig. 4

From: APOC3 induces endothelial dysfunction through TNF-α and JAM-1

Fig. 4

The expression of JAM-1 in HUVECs in protein level. A FCM results. Blank: Untreated HUVECS; APOC3: HUVECs that were incubated with APOC3 (100 μg/ml) for 24 h; APOC3 + siTNF-α: HUVECs that were transfected with TNF-α siRNA before they were incubated with APOC3 (100 μg/ml) for 24 h. Each bar represents the mean ± SE of 3 independent experiments. ** P < 0.01, significantly different from untreated HUVECs; ## P < 0.01, significantly different from HUVECs that were incubated with APOC3. B immunofluorescence results. a Untreated HUVECS; b HUVECs that were incubated with APOC3 (100 μg/ml) for 24 h; c HUVECs that were transfected with TNF-α siRNA before they were incubated with APOC3 (100 μg/ml) for 24 h; d relative expression levels of JAM-1 in HUVECS. Blank: Untreated HUVECS; APOC3: HUVECs that were incubated with APOC3 (100 μg/ml) for 24 h; APOC3 + siTNF-α: HUVECs that were transfected with TNF-α siRNA before they were incubated with APOC3 (100 μg/ml) for 24 h. Each bar represents the mean ± SE of 3 independent experiments. ** P < 0.01, significantly different from untreated HUVECs; * P < 0.05, significantly different from untreated HUVECs; # P < 0.05, significantly different from HUVECs that were incubated with APOC3

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