Fig. 2

Epo-R signaling after acute rHuEpo administration. Western blot analysis for activation of relevant signaling proteins downstream of the Epo-R. Phosphorylation levels are measured 1 h post placebo/rHuEpo treatment, and normalized to the total levels of the given protein. The median and 25 % and 75 % percentiles are shown for placebo (white bar) and rHuEpo treatment (gray bars). Two subjects were omitted from the analysis due to inadequate homogenization, thus, n = 8. No statistical significant differences in phosphorylation levels were detected between treatments using a Wilcoxon signed-rank test