Fig. 3

Lipolytic pathways after acute rHuEpo administration. Western blot analysis evaluating activation of different proteins involved in lipolysis. Phosphorylation levels are measured 1 h post placebo/rHuEpo treatment, and normalized to the total levels of the given protein. The median and 25 % and 75 % percentiles are shown for placebo (white bar) and rHuEpo treatment (gray bars). Two subjects were omitted from the analysis due to inadequate homogenization, and 1–2 subjects because of shortage of homogenate, thus, n = 6–7. No statistical significant differences in phosphorylation levels or total protein levels were detected between treatments using a Wilcoxon signed-rank test