Table 2 Effect of seed pre-treatment processing techniques on fatty acids composition of chia seed oil at different storage intervals

Storage Day 0

6.76 ± 0.15^c

6.85 ± 0.16^c

6.92 ± 0.11^c

6.95 ± 0.10^c

7.07 ± 0.13^d

7.11 ± 0.11^d

7.23 ± 0.19^d

7.32 ± 0.18^d

Storage Day 30 (25 ± 2 °C)

7.45 ± 0.13^b

7.67 ± 0.18^b

7.79 ± 0.13^b

7.88 ± 0.12^b

8.12 ± 0.15^b

8.22 ± 0.12^b

8.28 ± 0.17^b

8.52 ± 0.19^b

Storage Day 30 (4 ± 1 °C)

6.94 ± 0.14^c

7.05 ± 0.20^c

7.15 ± 0.15^c

7.23 ± 0.14^c

7.46 ± 0.16^c

7.53 ± 0.13^c

7.68 ± 0.18^c

7.79 ± 0.14^c

Storage Day 60 (25 ± 2 °C)

8.76 ± 0.18^a

8.82 ± 0.14^a

8.95 ± 0.17^a

9.09 ± 0.16^a

9.32 ± 0.17^a

9.41 ± 0.14^a

9.65 ± 0.16^a

9.81 ± 0.13^a

Storage Day 60 (4 ± 1 °C)

7.54 ± 0.17^b

7.57 ± 0.12^b

7.71 ± 0.19^b

7.87 ± 0.18^b

8.04 ± 0.19^b

8.12 ± 0.16^b

8.23 ± 0.15^b

8.41 ± 0.11^b

Storage Day 0

2.78 ± 0.07^c

2.85 ± 0.08^c

2.91 ± 0.09^c

2.98 ± 0.10^c

3.11 ± 0.06^d

3.24 ± 0.11^d

3.31 ± 0.09^d

3.44 ± 0.11^d

Storage Day 30 (25 ± 2 °C)

3.54 ± 0.09^b

3.61 ± 0.10^b

3.76 ± 0.11^b

3.87 ± 0.11^b

3.98 ± 0.07^b

4.16 ± 0.12^b

4.24 ± 0.08^b

4.31 ± 0.09^b

Storage Day 30 (4 ± 1 °C)

2.95 ± 0.10^c

2.99 ± 0.11^c

3.14 ± 0.12^c

3.28 ± 0.13^c

3.45 ± 0.09^c

3.67 ± 0.14^c

3.81 ± 0.07^c

3.98 ± 0.11^c

Storage Day 60 (25 ± 2 °C)

4.26 ± 0.11^a

4.33 ± 0.13^a

4.56 ± 0.14^a

4.82 ± 0.12^a

5.04 ± 0.08^a

5.15 ± 0.15^a

5.30 ± 0.06^a

5.51 ± 0.12^a

Storage Day 60 (4 ± 1 °C)

3.36 ± 0.12^b

3.47 ± 0.14^b

3.58 ± 0.13^b

3.74 ± 0.11^b

3.92 ± 0.10^b

4.10 ± 0.17^b

4.23 ± 0.11^b

4.35 ± 0.13^b

Storage Day 0

8.34 ± 0.19^d

8.56 ± 0.16^d

8.62 ± 0.15^d

8.69 ± 0.13^d

8.89 ± 0.11^d

9.05 ± 0.14^d

9.26 ± 0.16^d

9.38 ± 0.21^d

Storage Day 30 (25 ± 2 °C)

9.12 ± 0.16^b

9.23 ± 0.15^b

9.36 ± 0.13^b

9.49 ± 0.14^b

9.73 ± 0.13^b

9.89 ± 0.15^b

10.05 ± 0.19^b

10.23 ± 0.19^b

Storage Day 30 (4 ± 1 °C)

8.72 ± 0.15^c

8.84 ± 0.14^c

8.98 ± 0.11^c

9.16 ± 0.16^c

9.27 ± 0.12^c

9.43 ± 0.17^c

9.56 ± 0.17^c

9.78 ± 0.17^c

Storage Day 60 (25 ± 2 °C)

10.45 ± 0.14^a

10.66 ± 0.18^a

10.88 ± 0.12^a

11.37 ± 0.15^a

11.52 ± 0.14^a

11.69 ± 0.15^a

11.75 ± 0.18^a

11.92 ± 0.16^a

Storage Day 60 (4 ± 1 °C)

9.09 ± 0.13^b

9.20 ± 0.16^b

9.42 ± 0.14^b

9.73 ± 0.12^b

9.86 ± 0.15^b

9.94 ± 0.19^b

10.16 ± 0.19^b

10.38 ± 0.15^b

Storage Day 0

12.14 ± 0.22^a

12.03 ± 0.26^a

11.94 ± 0.22^a

11.81 ± 0.24^a

11.79 ± 0.32^a

11.62 ± 0.27^a

11.45 ± 0.22^a

11.28 ± 0.29^a

Storage Day 30 (25 ± 2 °C)

9.37 ± 0.24^c

9.25 ± 0.32^c

9.09 ± 0.23^c

8.93 ± 0.26^c

8.76 ± 0.34^c

8.54 ± 0.22^c

8.32 ± 0.25^c

8.13 ± 0.31^c

Storage Day 30 (4 ± 1 °C)

10.44 ± 0.20^b

10.29 ± 0.30^b

10.03 ± 0.25^b

9.86 ± 0.28^b

9.67 ± 0.26^b

9.65 ± 0.25^b

9.42 ± 0.32^b

9.15 ± 0.34^b

Storage Day 60 (25 ± 2 °C)

8.66 ± 0.23^d

8.55 ± 0.27^d

8.13 ± 0.29^d

8.01 ± 0.31^d

7.91 ± 0.27^d

7.83 ± 0.20^d

7.45 ± 0.36^d

7.26 ± 0.30^d

Storage Day 60 (4 ± 1 °C)

9.46 ± 0.25^c

9.31 ± 0.28^c

9.16 ± 0.24^c

9.04 ± 0.33^c

8.84 ± 0.29^c

8.68 ± 0.22^c

8.36 ± 0.23^c

8.03 ± 0.25^c

Storage Day 0

60.56 ± 1.22^a

60.43 ± 1.12^a

60.14 ± 1.20^a

59.99 ± 1.12^a

59.73 ± 1.17^a

59.45 ± 1.53^a

59.26 ± 1.34^a

58.84 ± 1.39^a

Storage Day 30 (25 ± 2 °C)

55.42 ± 1.34^c

55.13 ± 1.21^c

54.92 ± 1.15^c

54.43 ± 1.15^c

54.24 ± 1.19^c

53.88 ± 1.36^c

53.41 ± 1.45^c

52.61 ± 1.27^c

Storage Day 30 (4 ± 1 °C)

58.6 ± 1.36^b

58.24 ± 1.25^b

57.98 ± 1.17^b

57.19 ± 1.25^b

56.4 ± 1.21^b

55.60 ± 1.42^b

54.82 ± 1.37^b

53.42 ± 1.35^b

Storage Day 60 (25 ± 2 °C)

52.7 ± 1.28^d

52.34 ± 1.29^d

51.93 ± 1.24^d

51.48 ± 1.22^d

50.87 ± 1.25^e

49.94 ± 1.26^e

49.22 ± 1.39^e

48.52 ± 1.44^e

Storage Day 60 (4 ± 1 °C)

56.3 ± 1.14^c

55.96 ± 1.33^c

55.19 ± 1.27^c

54.81 ± 1.28^c

53.36 ± 1.28^d

52.76 ± 1.48^d

52.14 ± 1.41^d

51.63 ± 1.48^d