Fig. 2

Changes in expression of Ki-67, subcellular localization of PPARα and expression of cell cycle regulators. a Ki-67 is a marker of cell proliferation which is independent on specific phase of cell cycle (G1, S, G2, M). Increased number of Ki-67 positive cells was detected after treatment at maximal viability concentrations of used fibrates. These results confirm increased viability after fibrate treatment determined by WST-1 test. b In all three tested cell lines, we detected an increased number of cells with nuclear positivity of PPARα after fibrates treatment. Both, cytplasmic and nuclear positivity, is apparent. c Expression of cyclin E is increased in HEK293, HepG2, and HT-29 cell lines after fibrate treatment. d Expression of cyclin A is increased in carcinoma cell lines HepG2, and HT-29. In HEK293 cell line, there is slight decrease in cells treated by maximal viability concentrations of fibrates. e Expression of Cdc25A is decreased in HEK293, HepG2, and HT-29 cell lines after fibrate treatment. Graphs display results as fold changes. * Statistically different from control value at p < 0.05. Microphotographs (magn. 400×) show expression of proteins of interest in control cells (treated by 0.1 % DMSO)