Fig. 5
From: Acid sphingomyelinase as target of Lycium Chinense: promising new action for cell health
Effect of LC berry extract on gene and protein expression of HepG2 cells. The study was performed in control (without LC) and experimental cells (with LC) collected after 24 h of culture. a RTqPCR analysis of a panel of 96 genes involved in oxidative stress, proliferation, apoptosis and cancer was used. The results are normalized with the levels of the GAPDH and express as mRNA of experimental sample versus control sample. b immunoblotting analysis, the position of the 15 kDa protein for GLRX2, 13 kDa for RNF7, 69 kDa for PTGS1, and 48 kDa for beta-tubulin is indicated in relation to the position of molecular size standards. c area density is evaluated by densitometry scanning and analyzed with Scion Image. The results of GLRX2 and RNF7 are normalized with the levels of beta-tubulin. Data are expressed as the mean ± S.D. of 3 independent experiments performed in threeplicates. (Significance, *P < 0.001 versus control sample)