Table 1 Sequence information on the primers used for RT-PCR
From: A possible regulatory link between Twist 1 and PPARγ gene regulation in 3T3-L1 adipocytes
Genes | Sequences | Product size (bp) | Annealing temperature (°C) | Gene Bank |
|---|---|---|---|---|
Mouse Twist 1 | 5′-CATGGCTAACGTGCGGGA-3′ | 124 | 60 | NM_011658.2 |
5′-CGCCAGTTTGAGGGTCTGAA-3′ | ||||
Mouse PPARγ | 5′-GCTGACCCAATGGTTGCTGA-3′ | 181 | 60.5 | NM_001127330.2 |
5′-CTTTATCCCCACAGACTCGGC-3′ | ||||
GAPDH | 5′-TGGCCTTCCGTGTTCCTAC-3′ | 178 | 59 | NM_001289726.1 |
5′-GAGTTGCTGTTGAAGTCGCA-3′ |