Fig. 5

Fluorescence microscopy images of Tetraselmis sp. grown under different conditions. (a, b and c) correspond to fluorescence images of cells stained with NR (Excitation wavelength centered at 555 nm and fluorescence emission wavelength centered at 600 nm) to highlight the lipid droplets (red) cultured under indicated conditions. (a’, b’ and c’) correspond to phase contrast images of corresponding culture conditions. (a and a’) culture condition 7. (b and b’) culture condition 4. (c and c’) culture condition 5. All images of epifluorescence microscopy were taken at × 20 magnification, while contrast images were taken at × 40 magnification. Scale bar represents 20 μm