Skip to main content

Advertisement

Table 3 Oxidative stress parameters in SHR, SHR-Dca+/− and SHR-Dca−/− male rats

From: Heterozygous connexin 50 mutation affects metabolic syndrome attributes in spontaneously hypertensive rat

Phenotype SHR SHR-Dca+/− SHR-Dca−/− p-value
Plasma SOD 0.343 ± 0.030 0.319 ± 0.030 0.283 ± 0.034 0.43
Liver SOD 0.097 ± 0.006 0.074 ± 0.005† 0.082 ± 0.003* 0.012
Kidney SOD 0.070 ± 0.002 0.040 ± 0.002‡,b 0.059 ± 0.008 0.0010
Heart SOD 0.085 ± 0.008 0.079 ± 0.009 0.083 ± 0.009 0.88
Plasma CAT 341 ± 16 354 ± 30 414 ± 55 0.36
Liver CAT 773 ± 37 1081 ± 24‡,c 827 ± 31 0.000004
Kidney CAT 464 ± 19 571 ± 41 539 ± 46 0.16
Heart CAT 629 ± 36 679 ± 31 621 ± 38 0.44
Plasma GSH-Px 546 ± 30 379 ± 27† 426 ± 60 0.027
Liver GSH-Px 417 ± 27 320 ± 18* 389 ± 33 0.042
Kidney GSH-Px 506 ± 31 378 ± 23†,a 460 ± 24 0.0092
Heart GSH-Px 624 ± 35 577 ± 20 612 ± 16 0.38
Plasma GR 212 ± 21 158 ± 13*,c 259 ± 13 0.001
Liver GR 387 ± 22 430 ± 14 372 ± 32 0.20
Kidney GR 273 ± 16 241 ± 9 198 ± 31 0.06
Heart GR 244 ± 24 228 ± 16 192 ± 26 0.27
Plasma GSH 31.0 ± 2.0 22.3 ± 1.1‡ 21.9 ± 1.0‡ 0.0004
Liver GSH 24.6 ± 1.5 23.4 ± 1.3 28.2 ± 1.8 0.10
Kidney GSH 25.1 ± 1.7 19.8 ± 1.2 24.6 ± 1.9 0.06
Heart GSH 25.8 ± 2.4 27.4 ± 2.0 25.5 ± 1.2 0.75
Plasma TBARS 1.72 ± 0.05 2.32 ± 0.13† 2.24 ± 0.13† 0.0030
Liver TBARS 0.62 ± 0.04 1.05 ± 0.09‡ 0.87 ± 0.05* 0.0016
Kidney TBARS 1.27 ± 0.11 1.49 ± 0.14 1.54 ± 0.16 0.37
Heart TBARS 1.88 ± 0.11 2.38 ± 0.23 1.97 ± 0.23 0.20
Plasma CD 31.6 ± 2.7 34.8 ± 1.5 33.0 ± 2.0 0.54
Liver CD 33.7 ± 1.0 32.1 ± 1.6 29.1 ± 2.2 0.18
Kidney CD 19.8 ± 2.3 20.7 ± 1.6 20.8 ± 2.3 0.93
Heart CD 15.1 ± 1.1 19.5 ± 1.4 19.1 ± 1.9 0.11
  1. The parameters of oxidative stress in plasma, liver, kidney cortex and heart of SHR, SHR-Dca+/− and SHR-Dca−/− adult male rats are shown as mean ± SEM. The significance levels of one-way ANOVA for STRAIN as a major factor are shown in the last column; significant values are italicized. * p < 0.05, † p < 0.01 and ‡ p < 0.001 for pair-wise comparisons (post-hoc Tukey’s HSD test) between SHR-Dca−/− or SHR-Dca+/− strains vs. SHR. a p < 0.05, b p < 0.01 and c p < 0.001 for pair-wise comparisons between SHR-Dca−/− and SHR-Dca+/− strains. Superoxide dismutase (SOD) units : U I ml−1 (plasma), U I mg prot−1 (liver, kidney, heart); Catalase (CAT) units: μM H2O2 min−1 ml−1 (plasma), μM H2O2 min−1mg prot−1 (liver, kidney, heart); Glutathione peroxidase (GSH-Px) units μM GSH min−1ml−1 (plasma), μM GSH min−1mg prot−1 (liver, kidney, heart); Glutathione reductase (GR) units: nM NADPH min−1ml−1 (plasma), nM NADPH min−1mg prot−1 (liver, kidney, heart); Glutathione (GSH) units: nM ml−1 (plasma), μM mg prot−1 (liver, kidney, heart); Thiobarbituric acid reactive substances (TBARS) units nM ml−1 (plasma), nM mg prot−1 (liver, kidney, heart); conjugated dienes (CD) units: nM ml−1 (plasma), nM mg prot−1 (liver, kidney, heart). * p < 0.05 vs. SHR. a p < 0.05 vs. SHR-Dca−/−.p < 0.01 vs. SHR. b p < 0.01 vs. SHR-Dca−/−.p < 0.001 vs. SHR. c p < 0.001 vs. SHR-Dca−/−