|
Phenotype
|
SHR
|
SHR-Dca+/−
|
SHR-Dca−/−
|
p-value
|
|---|
|
Plasma SOD
|
0.343 ± 0.030
|
0.319 ± 0.030
|
0.283 ± 0.034
|
0.43
|
|
Liver SOD
|
0.097 ± 0.006
|
0.074 ± 0.005†
|
0.082 ± 0.003*
|
0.012
|
|
Kidney SOD
|
0.070 ± 0.002
|
0.040 ± 0.002‡,b
|
0.059 ± 0.008
|
0.0010
|
|
Heart SOD
|
0.085 ± 0.008
|
0.079 ± 0.009
|
0.083 ± 0.009
|
0.88
|
|
Plasma CAT
|
341 ± 16
|
354 ± 30
|
414 ± 55
|
0.36
|
|
Liver CAT
|
773 ± 37
|
1081 ± 24‡,c
|
827 ± 31
|
0.000004
|
|
Kidney CAT
|
464 ± 19
|
571 ± 41
|
539 ± 46
|
0.16
|
|
Heart CAT
|
629 ± 36
|
679 ± 31
|
621 ± 38
|
0.44
|
|
Plasma GSH-Px
|
546 ± 30
|
379 ± 27†
|
426 ± 60
|
0.027
|
|
Liver GSH-Px
|
417 ± 27
|
320 ± 18*
|
389 ± 33
|
0.042
|
|
Kidney GSH-Px
|
506 ± 31
|
378 ± 23†,a
|
460 ± 24
|
0.0092
|
|
Heart GSH-Px
|
624 ± 35
|
577 ± 20
|
612 ± 16
|
0.38
|
|
Plasma GR
|
212 ± 21
|
158 ± 13*,c
|
259 ± 13
|
0.001
|
|
Liver GR
|
387 ± 22
|
430 ± 14
|
372 ± 32
|
0.20
|
|
Kidney GR
|
273 ± 16
|
241 ± 9
|
198 ± 31
|
0.06
|
|
Heart GR
|
244 ± 24
|
228 ± 16
|
192 ± 26
|
0.27
|
|
Plasma GSH
|
31.0 ± 2.0
|
22.3 ± 1.1‡
|
21.9 ± 1.0‡
|
0.0004
|
|
Liver GSH
|
24.6 ± 1.5
|
23.4 ± 1.3
|
28.2 ± 1.8
|
0.10
|
|
Kidney GSH
|
25.1 ± 1.7
|
19.8 ± 1.2
|
24.6 ± 1.9
|
0.06
|
|
Heart GSH
|
25.8 ± 2.4
|
27.4 ± 2.0
|
25.5 ± 1.2
|
0.75
|
|
Plasma TBARS
|
1.72 ± 0.05
|
2.32 ± 0.13†
|
2.24 ± 0.13†
|
0.0030
|
|
Liver TBARS
|
0.62 ± 0.04
|
1.05 ± 0.09‡
|
0.87 ± 0.05*
|
0.0016
|
|
Kidney TBARS
|
1.27 ± 0.11
|
1.49 ± 0.14
|
1.54 ± 0.16
|
0.37
|
|
Heart TBARS
|
1.88 ± 0.11
|
2.38 ± 0.23
|
1.97 ± 0.23
|
0.20
|
|
Plasma CD
|
31.6 ± 2.7
|
34.8 ± 1.5
|
33.0 ± 2.0
|
0.54
|
|
Liver CD
|
33.7 ± 1.0
|
32.1 ± 1.6
|
29.1 ± 2.2
|
0.18
|
|
Kidney CD
|
19.8 ± 2.3
|
20.7 ± 1.6
|
20.8 ± 2.3
|
0.93
|
|
Heart CD
|
15.1 ± 1.1
|
19.5 ± 1.4
|
19.1 ± 1.9
|
0.11
|
- The parameters of oxidative stress in plasma, liver, kidney cortex and heart of SHR, SHR-Dca+/− and SHR-Dca−/− adult male rats are shown as mean ± SEM. The significance levels of one-way ANOVA for STRAIN as a major factor are shown in the last column; significant values are italicized. * p < 0.05, † p < 0.01 and ‡ p < 0.001 for pair-wise comparisons (post-hoc Tukey’s HSD test) between SHR-Dca−/− or SHR-Dca+/− strains vs. SHR. a
p < 0.05, b
p < 0.01 and c
p < 0.001 for pair-wise comparisons between SHR-Dca−/− and SHR-Dca+/− strains. Superoxide dismutase (SOD) units : U I ml−1 (plasma), U I mg prot−1 (liver, kidney, heart); Catalase (CAT) units: μM H2O2 min−1 ml−1 (plasma), μM H2O2 min−1mg prot−1 (liver, kidney, heart); Glutathione peroxidase (GSH-Px) units μM GSH min−1ml−1 (plasma), μM GSH min−1mg prot−1 (liver, kidney, heart); Glutathione reductase (GR) units: nM NADPH min−1ml−1 (plasma), nM NADPH min−1mg prot−1 (liver, kidney, heart); Glutathione (GSH) units: nM ml−1 (plasma), μM mg prot−1 (liver, kidney, heart); Thiobarbituric acid reactive substances (TBARS) units nM ml−1 (plasma), nM mg prot−1 (liver, kidney, heart); conjugated dienes (CD) units: nM ml−1 (plasma), nM mg prot−1 (liver, kidney, heart). * p < 0.05 vs. SHR.
a
p < 0.05 vs. SHR-Dca−/−. † p < 0.01 vs. SHR.
b
p < 0.01 vs. SHR-Dca−/−. ‡ p < 0.001 vs. SHR.
c
p < 0.001 vs. SHR-Dca−/−
