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Table 3 Oxidative stress parameters in SHR, SHR-Dca+/− and SHR-Dca−/− male rats

From: Heterozygous connexin 50 mutation affects metabolic syndrome attributes in spontaneously hypertensive rat

Phenotype

SHR

SHR-Dca+/−

SHR-Dca−/−

p-value

Plasma SOD

0.343 ± 0.030

0.319 ± 0.030

0.283 ± 0.034

0.43

Liver SOD

0.097 ± 0.006

0.074 ± 0.005†

0.082 ± 0.003*

0.012

Kidney SOD

0.070 ± 0.002

0.040 ± 0.002‡,b

0.059 ± 0.008

0.0010

Heart SOD

0.085 ± 0.008

0.079 ± 0.009

0.083 ± 0.009

0.88

Plasma CAT

341 ± 16

354 ± 30

414 ± 55

0.36

Liver CAT

773 ± 37

1081 ± 24‡,c

827 ± 31

0.000004

Kidney CAT

464 ± 19

571 ± 41

539 ± 46

0.16

Heart CAT

629 ± 36

679 ± 31

621 ± 38

0.44

Plasma GSH-Px

546 ± 30

379 ± 27†

426 ± 60

0.027

Liver GSH-Px

417 ± 27

320 ± 18*

389 ± 33

0.042

Kidney GSH-Px

506 ± 31

378 ± 23†,a

460 ± 24

0.0092

Heart GSH-Px

624 ± 35

577 ± 20

612 ± 16

0.38

Plasma GR

212 ± 21

158 ± 13*,c

259 ± 13

0.001

Liver GR

387 ± 22

430 ± 14

372 ± 32

0.20

Kidney GR

273 ± 16

241 ± 9

198 ± 31

0.06

Heart GR

244 ± 24

228 ± 16

192 ± 26

0.27

Plasma GSH

31.0 ± 2.0

22.3 ± 1.1‡

21.9 ± 1.0‡

0.0004

Liver GSH

24.6 ± 1.5

23.4 ± 1.3

28.2 ± 1.8

0.10

Kidney GSH

25.1 ± 1.7

19.8 ± 1.2

24.6 ± 1.9

0.06

Heart GSH

25.8 ± 2.4

27.4 ± 2.0

25.5 ± 1.2

0.75

Plasma TBARS

1.72 ± 0.05

2.32 ± 0.13†

2.24 ± 0.13†

0.0030

Liver TBARS

0.62 ± 0.04

1.05 ± 0.09‡

0.87 ± 0.05*

0.0016

Kidney TBARS

1.27 ± 0.11

1.49 ± 0.14

1.54 ± 0.16

0.37

Heart TBARS

1.88 ± 0.11

2.38 ± 0.23

1.97 ± 0.23

0.20

Plasma CD

31.6 ± 2.7

34.8 ± 1.5

33.0 ± 2.0

0.54

Liver CD

33.7 ± 1.0

32.1 ± 1.6

29.1 ± 2.2

0.18

Kidney CD

19.8 ± 2.3

20.7 ± 1.6

20.8 ± 2.3

0.93

Heart CD

15.1 ± 1.1

19.5 ± 1.4

19.1 ± 1.9

0.11

  1. The parameters of oxidative stress in plasma, liver, kidney cortex and heart of SHR, SHR-Dca+/− and SHR-Dca−/− adult male rats are shown as mean ± SEM. The significance levels of one-way ANOVA for STRAIN as a major factor are shown in the last column; significant values are italicized. * p < 0.05, † p < 0.01 and ‡ p < 0.001 for pair-wise comparisons (post-hoc Tukey’s HSD test) between SHR-Dca−/− or SHR-Dca+/− strains vs. SHR. a p < 0.05, b p < 0.01 and c p < 0.001 for pair-wise comparisons between SHR-Dca−/− and SHR-Dca+/− strains. Superoxide dismutase (SOD) units : U I ml−1 (plasma), U I mg prot−1 (liver, kidney, heart); Catalase (CAT) units: μM H2O2 min−1 ml−1 (plasma), μM H2O2 min−1mg prot−1 (liver, kidney, heart); Glutathione peroxidase (GSH-Px) units μM GSH min−1ml−1 (plasma), μM GSH min−1mg prot−1 (liver, kidney, heart); Glutathione reductase (GR) units: nM NADPH min−1ml−1 (plasma), nM NADPH min−1mg prot−1 (liver, kidney, heart); Glutathione (GSH) units: nM ml−1 (plasma), μM mg prot−1 (liver, kidney, heart); Thiobarbituric acid reactive substances (TBARS) units nM ml−1 (plasma), nM mg prot−1 (liver, kidney, heart); conjugated dienes (CD) units: nM ml−1 (plasma), nM mg prot−1 (liver, kidney, heart). * p < 0.05 vs. SHR. a p < 0.05 vs. SHR-Dca−/−.p < 0.01 vs. SHR. b p < 0.01 vs. SHR-Dca−/−.p < 0.001 vs. SHR. c p < 0.001 vs. SHR-Dca−/−