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Table 3 Experimental results of Box-Behnken design

From: Effects of nutritional conditions on growth and biochemical composition of Tetraselmis sp.

Run No

Factors

Responses

NaNO3 (mM)

NaH2PO4 (mM)

Metals (μL.L−1)

Vitamins (μL.L−1)

Biomass (g.L−1)

Total chlorophylls content (mg.g DW−1)

Carotenoids content (mg.g DW−1)

Starch content (g.g DW−1)

1

0.58

0.018

1000

375

2.2

0.72

0.13

0.38

2

1.76

0.018

1000

375

3.28

2.5

0.08

0.12

3

0.58

0.054

1000

375

1.19

3.7

0.2

0.25

4

1.76

0.054

1000

375

1.7

4.62

0.28

0.3

5

1.17

0.036

500

250

3.37

2.04

0.06

0.38

6

1.17

0.036

1500

250

4.57

1.15

0.12

0.26

7

1.17

0.036

500

500

3.14

3.32

0.15

0.32

8

1.17

0.036

1500

500

2.15

3.69

0.16

0.32

9

1.17

0.036

1000

375

2.6

3.59

0.17

0.32

10

0.58

0.036

1000

250

1.95

1.41

0.17

0.42

11

1.76

0.036

1000

250

2.91

2.5

0.18

0.23

12

0.58

0.036

1000

500

1.77

2.5

0.24

0.29

13

1.76

0.036

1000

500

2.1

4.5

0.23

0.33

14

1.17

0.018

500

375

3.39

1.7

0.09

0.29

15

1.17

0.054

500

375

2.61

3.48

0.1

0.33

16

1.17

0.018

1500

375

4.8

1.3

0.05

0.36

17

1.17

0.054

1500

375

1.72

3.5

0.26

0.3

18

1.17

0.036

1000

375

2.37

3.71

0.17

0.33

19

0.58

0.036

500

375

1.94

2.3

0.16

0.37

20

1.76

0.036

500

375

1.62

3.7

0.21

0.31

21

0.58

0.036

1500

375

1.85

2

0.18

0.33

22

1.76

0.036

1500

375

2.55

3.61

0.2

0.25

23

1.17

0.018

1000

250

5.2

0.8

0.04

0.28

24

1.17

0.054

1000

250

2.1

3.38

0.19

0.38

25

1.17

0.018

1000

500

1.99

2.42

0.16

0.32

26

1.17

0.054

1000

500

2.2

4.92

0.23

0.28

27

1.17

0.036

1000

375

2.15

3.72

0.19

0.37