Fig. 3
From: Berberine increases adipose triglyceride lipase in 3T3-L1 adipocytes through the AMPK pathway
On D12, cells were treated with 10 μM and 100 μM BBR for 24 h or 48 h in serum-starved DMEM. a Proteins were separated by SDS-PAGE and immunoblotted for p-HSL (Ser 565), HSL, ATGL, GPAT3 and β-actin. The values of p-HSL were quantified using densitometry and normalized with HSL, while those of ATGL and GPAT3 were quantified by densitometry and normalized with β-actin. Representative Western blot results are shown. b Total RNA was extracted from differentiated cells treated with 10 μM and 100 μM BBR for 24 h or 48 h in serum-free DMEM. mRNA levels of ATGL and HSL were determined by real-time PCR and normalized with β-actin. Values are reported as the fold-change relative to the control group. The data are from 3 independent experiments and are presented as the mean ± SD. * p <0.05 versus the control group, **p <0.01 versus the control group