Fig. 4
From: Berberine increases adipose triglyceride lipase in 3T3-L1 adipocytes through the AMPK pathway
a On D12, cells were treated with 10 μM and 100 μM BBR for 24 h in serum-starved DMEM. Proteins were separated by SDS-PAGE and immunoblotted for p-AMPK, AMPK and β-actin. The values of p-AMPK were quantified using densitometry and normalized with AMPK. Representative Western blot results are shown. b On D12, the cells were stimulated with 20 μM Compound C or 10 μM BBR. After 1 h, the cells from one of the two dishes pretreated with Compound C were exposed to 10 μM BBR. All cells were treated for 24 h in serum-free DMEM. Proteins were separated by SDS-PAGE and immunoblotted for p-HSL, HSL, ATGL and β-actin. Values of p-HSL were quantified by densitometry and normalized with HSL while those of ATGL were quantified by densitometry and normalized with β-actin. Representative Western blot results are shown. Values are reported as the fold-change relative to the control group. c The visualization of ATGL by immunofluorescence staining (red). Nuclei were stained with DAPI (blue). Values are reported as the fold-change relative to the control group. The data are from 3 independent experiments and are presented as the mean ± SD. *p <0.05 versus the control group, **p <0.01 versus the control group. Original magnification: 400 ×