Fig. 2
From: Differential effects of palmitoleic acid on human lymphocyte proliferation and function
Effect of palmitoleic acid on human lymphocyte DNA fragmentation. After treatment with 12.5 to 100 μM of PA for 24 h, cells were stained with a buffer containing citrate, Triton X100 and propidium iodite and analyzed by flow cytometry. The percentages correspond to the fraction of cells with fragmented DNA. Representative dot plots of the forward-light scatter (x axis) and side-light scatter (y axis) used as indicators of cell size and granularity respectively, and histograms from PI fluorescence are shown in: a Control cells incubated only with RPMI medium; b Cells treated with 0.05% of ethanol; c Cells treated with 50 μM of PA; d Cells treated with 75 μM of PA. The values are presented in the graph below as mean ± S.E.M. of three determinations from six experiments. *P < 0.01 versus control or ethanol