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Fig. 4 | Lipids in Health and Disease

Fig. 4

From: Differential effects of palmitoleic acid on human lymphocyte proliferation and function

Fig. 4

Effect of palmitoleic acid (PA) and oleic acid (OA) at non-toxic concentration on human lymphocyte CD28 membrane surface expression. Cells were incubated with 25 and 50 μM of PA or OA in the presence or absence of ConA for 24 h. After, cells were stained with anti- PerCP-Cy5- CD28 and analyzed by flow cytometry. Negative controls were incubated with non-reactive IgG. Representative dot plots of the forward-light scatter (x axis) and side-light scatter (y axis) used as indicators of cell size and granularity respectively, and histograms from PerCP-Cy5 fluorescence (related to CD28 detection) are shown in: a Negative controls; b ConA stimulated control cells; c 25 μM PA + ConA treated cells; d 25 μM OA + ConA treated cells. The values are presented in the graph below as mean ± S.E.M. of three determinations from six experiments. *P < 0.01 versus control. ♦P < 0.05 versus 25 μM of OA. •P < 0.05 versus 50 μM of OA

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