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Fig. 3 | Lipids in Health and Disease

Fig. 3

From: Butyrate attenuates lipolysis in adipocytes co-cultured with macrophages through non-prostaglandin E2–mediated and prostaglandin E2–mediated pathways

Fig. 3

Effects of butyrate on COX2 protein and mRNA expression in RAW264.7 (a, b, c) and 3T3-L1 (d, e, f) cells co-cultured using the transwell method for 24 h. COX2 and actin proteins were detected by western blotting (a, d). The expression ratio of COX2 to actin was determined based on quantification of the relative density of western blotting bands (b, e). COX2 and β-actin mRNAs were detected using qRT-PCR (c, f), as described in the Methods. For western blotting and protein expression, n = 3, and for qRT-PCR, n = 5. Values are expressed as the mean ± SD. *p < 0.05, **p < 0.01, and ***p < 0.001 versus the control, and # p < 0.05, ## p < 0.01, and ### p < 0.001 versus co-culture, as determined by ANOVA and the Tukey-Kramer test

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Lipids in Health and Disease

ISSN: 1476-511X