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Fig. 4 | Lipids in Health and Disease

Fig. 4

From: Butyrate attenuates lipolysis in adipocytes co-cultured with macrophages through non-prostaglandin E2–mediated and prostaglandin E2–mediated pathways

Fig. 4

Effects of butyrate on cPLA2 activity in RAW264.7 cells (a) and sPLA2 activity in RAW264.7 (b) and 3T3-L1 (c) cells co-cultured using the transwell method for 24 h. The activities of intracellular cPLA2 and sPLA2 were measured using an assay kit, as described in the Methods. As the control, activity was measured in non–co-cultured cells. Values from five independent experiments are expressed as mean ± SD. *p < 0.05, **p < 0.01, and ***p < 0.001 versus the control, and # p < 0.05 versus co-culture, as determined by ANOVA and the Tukey-Kramer test

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