Fig. 3

The effect of BPA on NPC1L1 expression in Caco-2 cells. a The cells were treated with BPA at different concentrations for 24 h, and the whole-cell lysates were analyzed by Western blot. The results are representative of three independent experiments. b NPC1L1 mRNA abundance was determined by real-time RT-PCR as described in Methods. Expression values were normalized to housekeeping genes, and expression in untreated cells was set to 1. Values shown represent means ± SEM of three independent experiments, *P < 0.05, **P < 0.01, compared to untreated cells. c After the blockade of NPC1L1 expression by ezetimibe(EZE), Caco-2 cells were incubated with BPA for 24 h, then incubated with radioactive micellar cholesterol for additional 2 h. The absorption of cholesterol in the absence of BPA was normalized to 100%. Results are mean ± SEM from triplicate determinations in three separate experiments. *P < 0.05 compared to untreated cell