Fig. 4
Time/dose response of Sch B treatment on epididymal adipose tissue (EAT) and adipocyte mass. Experimental details were described in Fig. 1. For the time course study, EAT index (EAT weight/body weight × 100) and adipocyte size were measured at 12, 24, 48, 72, 96, and 120 h after Sch B treatment (a, b and d). A representative microscopic picture of a haematoxylin and eosin (HE)-stained EAT section for each group is shown in (c). The adipocyte area of each section was computed by Image Proplus 6.0. For the dose–response study, mice were intragastrically treated with Sch B (0.25–2 g/kg). Twenty-four h later, the pharmacodynamic parameters (E max, K D, and pD 2) of Sch B on EAT index were measured (b). Values given are the mean ± SEM, with n = 10. *P < 0.05, **P < 0.01 vs Control, using a one-way ANOVA followed by Dunnett’s multiple comparisons test, Student’s t-test or post-hoc analysis