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Fig. 1 | Lipids in Health and Disease

Fig. 1

From: Pigment epithelium-derived factor alleviates endothelial injury by inhibiting Wnt/β-catenin pathway

Fig. 1

Ox-LDL induces cytotoxicity and oxidative stress in HUVECs. HUVECs were treated with ox-LDL at different concentrations (12.5, 25, 50, 100 and 150 mg/L) for 24 h. a Cell viability was measured by an MTT assay and results were expressed as the percentage of the control. b LDH activity in the medium was determined by an LDH assay kit. c Cell apoptosis was detected using flow cytometry, and the total apoptotic cells (early and late-stage apoptosis) are presented in the panel (Annexin V-FITC staining alone or together with PI). Intracellular ROS (d) generation was measured with the peroxide-sensitive fluorescent probe DCFH-DA using flow cytometry, and intracellular MDA level (e), SOD activity (f), and NO release (g) in the medium were detected with corresponding kits. The results are shown as the mean ± SD of at least 6 independent experiments. *P < 0.05, **P < 0.01 versus the control group

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