Fig. 4From: Pigment epithelium-derived factor alleviates endothelial injury by inhibiting Wnt/β-catenin pathwayWnt/β-catenin pathway mediates ox-LDL-induced oxidative stress in HUVECs. Cells were treated as described in Fig. 3 a The oxidation-sensitive fluorescent probe DCFH-DA was applied to assess intracellular ROS production by flow cytometry. MDA (b), SOD (c) and NO (d) levels were determined by commercial kits. The results are presented as the mean ± SD of at least 6 independent experiments. *P < 0.05, **P < 0.01 versus the control group; # P < 0.05, ## P < 0.01 versus the ox-LDL groupBack to article page