Fig. 5
From: Pigment epithelium-derived factor alleviates endothelial injury by inhibiting Wnt/β-catenin pathway
PEDF inhibits ox-LDL-induced cytotoxicity of HUVECs. HUVECs were pretreated with exogenous PEDF (50, 100, 200 and 400 ng/mL) for 24 h, or transfected with siRNA against PEDF and a negative control siRNA, followed by treatment with 100 mg/L ox-LDL for 24 h. A kit assay was performed to evaluate LDH activity (a) in the medium. Flow cytometry was used to analyze the ratio of apoptotic cells stained with Annexin V-FITC and PI (b). The silencing of PEDF was validated by western blotting (c) and quantitative real-time PCR (d). All results are presented as the mean ± SD of at least 6 independent experiments. *P < 0.05, **P < 0.01 versus the control group; # P < 0.05, ## P < 0.01 versus the ox-LDL group