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Fig. 2 | Lipids in Health and Disease

Fig. 2

From: Progranulin causes adipose insulin resistance via increased autophagy resulting from activated oxidative stress and endoplasmic reticulum stress

Fig. 2

Inhibition of iNOS reversed progranulin-induced ER stress response and increased autophagy, preventing from insulin resistance in adipocytes. a Protein expression of iNOS. b The relative protein quantity of iNOS. c Nitrate levels in the culture media. d Intracellular ROS levels. e Protein expression of CHOP and GRP78, and the phosphorylation of PERK. f The relative protein quantity of CHOP, GRP78 and p-PERK. g Protein expression of Atg7, p62 and LC3. h The relative protein quantity of Atg7, p62 and LC3. i IRS-1 tyrosine phosphorylation. j The relative protein quantity of IRS-1 tyrosine phosphorylation. k Glucose uptake. The relative quantity of proteins was analyzed using Quantity One software. A representative blot is shown and the data was expressed as mean ± SEM in each bar graph. *P < 0.05 (PGRN vs Control); # P < 0.05 (PGRN + SMT vs PGRN)

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