Docosahexaenoic acid increases accumulation of adipocyte triacylglycerol through up-regulation of lipogenic gene expression in pigs

Table 2 List of primer sets for quantitative PCR by the SYBR system

Gene Name	NCBI accession number	Forward primer (5’-3’)	Reverse primer (5’-3’)	Product size
ap2	NM_001002817.1	TGGTACAGGTGCAGAAGTGG	TTCTGGTAGCCGTGACACCT	108
ACOX1	NM_001101028.1	TTGGCCCCAAATTCGGCTAT	GGCTTCACCTGGGCATACTT	106
ADRP	NM_214200.2	GCTGGTGAGCAGTGGAGTAG	ACTTGGCTTCTGAACCATATCA	136
ATGL	NM_001098605.1	TTCCCCAAAGAGACGACGTG	CGGTGATGGTGCTCTTGAGT	196
β-actin^a	AY550069	GCCAGGTCATCACCATCGG	GTAGAGGTCCTTGCGGATGTC	103
CD36	NM_001044622.1	TGGGTTAAAACAGGCACGGA	TGCTACTTCCTCTGGATTCTGC	123
CGI-58	NM_001012407.1	TTTTCCTGAGCGACCAGACC	GTCCTGCTCCAAGAATGGCT	163
CPT1α	NM_001129805.1	AAGGACATGGGGAAGTTTTC	ACGTAGAGGCAGAACAGGTG	247
DGAT1	NM_214051.1	TTCTACAAGCCCATGCTCCG	AGAAGGCTGAAGCCAGGAAC	80
FAS	NM_001099930.1	GTGGGCTACAGCATGATAGG	GAATTGCAGCGAGGAGTTAG	189
HSL	NM_214315.1	CCCTCGGCTGTCAACTTCTT	GGTGCTAATCTCGTCTCGGG	102
LPL	NM_214286.1	TGGACGGTGACAGGAATGTA	AAGGCTGTATCCCAGGAGGT	72
MGL	NM_001143718.1	ACTTCTCCGGCATGGTTCTG	GGGACATGTTTGGCAGGACA	114
Perilipin	NM_001038638.1	CAACAAGGGCCTGACTTTGC	GTTGGCGGCATATTCAGCAG	190
PPARα	NM_001044526.1	CAGC CTC CAGC C CCTCGTC	GCGGTCTCGGCATCTTCTAGG	381
PPARγ	NM_214379.1	TCGCATCTTTCAGGGGTGTC	CCCAGGGATGTTCTTGGCAT	79
SREBP1	AY338729.1	CCACCAGTCCTGATGCCAG	GTACATCTTCAGCGGGGTGG	152

Abbreviations: ap2 adipocyte protein 2, ACOX1 acyl CoA oxidase, ADRP adipose differentiation related protein, ATGL adipose triglyceride lipase, CGI58 comparative gene identified-58, CPT1α carnitine palmitoyltransferase 1α, DGAT1 diglyceride acyltransferase 1, FAS fatty acid syntheses, HSL hormone sensitive lipase, LPL lipoprotein lipase, MGL monoglyceride lipase, PPARα peroxisome proliferator-activated receptor α, PPARγ peroxisome proliferator-activated receptor γ, SREBP1 sterol response element binding protein
^areference gene

ISSN: 1476-511X