Fig. 1

HDL containing ApoM protects endothelial cells against serum/GF deprivation-induced cell death. a HUVEC were grown to confluence in full medium and then switched to serum starvation medium or serum/GF deprivation medium. The graph represents the percentage of apoptotic cells (Annexin V⁺ and 7-ADD⁻) identified by flow cytometry. Error bars correspond to SEM of n = 5. One-way ANOVA p < 0.001 followed by Holm-Sidak method multiple-comparison post hoc test. b–d Cells were serum/GF deprived and treated with ± HDL^+ApoM 50 μg/ml or HDL^{-ApoM 50} μg/ml in b and c and ± Total HDL 500 μg/ml or HDL^-ApoM 500 μg/ml in d for 18 h and then analyzed by flow cytometry. Error bars correspond to SEM. c shows dot plots from a representative experiment of B. In b, n = 4, one-way ANOVA p = 0.001 followed by Holm-Sidak method multiple-comparison post hoc test. In d, n = 10, ANOVA on ranks p = 0.016 followed by SNK method multiple-comparison post hoc test. e-f Measurements of Caspase-3 activity in HUVEC lysates. Cells were incubated in serum/GF starvation medium with ± HDL^+ApoM 25 μg/ml, HDL^-ApoM 25 μg/ml for 24 h in e and for 2 h in f. Data were normalized versus serum/GF starvation condition and error bars correspond to SEM. In E, n = 4, ANOVA on ranks p = 0.006 followed by SNK method multiple-comparison post hoc test. In F, n = 3, one-way ANOVA p = 0.012 followed by Holm-Sidak method multiple-comparison post hoc test. *p < 0.05