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Fig. 3 | Lipids in Health and Disease

Fig. 3

From: HDL-associated ApoM is anti-apoptotic by delivering sphingosine 1-phosphate to S1P1 & S1P3 receptors on vascular endothelium

Fig. 3

Pharmacological activation of S1P1 or S1P3 protects endothelial cells against serum/GF deprivation-induced cell death. a Relative expression of S1PR in HUVEC. Total RNA was analyzed by qPCR using Taqman probes for S1PR and normalized against GAPDH expression. S1P1 expression was chosen as reference. Error bars correspond to SD, n.d., not detected. be HUVEC were grown up to confluence in full medium, then switched to serum/GF deprivation medium with SEW2971 5 μM b and e, ML-031 5 μM c and e or/and CYM5541 5 μM d and e for 18 h and then analyzed by flow cytometry. The percentage of apoptotic cells (AnnexinV+ and 7-ADD) was normalized versus serum/GF starvation condition. Error bars correspond to SEM. In b, n = 9, Mann-Whitney U-test p <0.001. In c, n = 7 Mann-Whitney U-test p = 0.026. In D, n = 4, Mann-Whitney U-test p = 0.343. In e, n = 5, one-way ANOVA p = 0.004 followed by Holm-Sidak multiple-comparison post hoc test. In e, * over the bars indicates statistical significance versus serum/GF deprivation treatment. *p < 0.05

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