Fig. 5

ApoM-bound S1P confers longer protection to endothelial cells against serum/GF deprivation. a–e Cells were treated with free S1P 1 μM, ApoM 1 μM, ApoM-bound S1P 1 μM or albumin-bound S1P 1 μM. a Cells were treated for 24 h and then a cell fragmentation assay performed. b Cells were treated for 2 h and then the Caspase 3 activity measured. ANOVA on ranks p < 0.001 followed by SNK multiple-comparison post hoc test. c As b, but 24 h treatment. ANOVA on ranks p = 0.007 followed by SNK multiple-comparison post hoc test. d Cell viability was determined by MTT after 24 h of treatment. Error bars correspond to SD. N = 4-5. One-way ANOVA p < 0.001 followed by Holm-Sidak method multiple-comparison post hoc test. e-f Cells were pre-incubated with ± W146 1 μM or CAY10444 10 μM in serum/GF deprivation medium for 30 min. and then treated as in c, but with the addition of W146 1 μM in e and CAY10444 10 μM in F. In E, n = 3, data were analyzed by one-way ANOVA p = 0.003 followed by Holm-Sidak method multiple-comparison post hoc test and in F, n = 3, by One-way ANOVA p < 0.001 followed by Holm-Sidak method multiple-comparison post hoc test. * over the bars indicates statistical significance versus serum/GF starvation treatment. *p < 0.05