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Fig. 6 | Lipids in Health and Disease

Fig. 6

From: HDL-associated ApoM is anti-apoptotic by delivering sphingosine 1-phosphate to S1P1 & S1P3 receptors on vascular endothelium

Fig. 6

AKT or ERK inhibition blocks S1P anti-apoptotic effect. a-b Cells were pre-incubated with ± W146 1 μM, CAY10444 10 μM, LY294002 50 μM, U0126 50 μM or PD98050 50 μM for 30 min and then treated with free S1P 1 μM, ApoM 1 μM, ApoM-bound S1P 1 μM or albumin-bound S1P 1 μM for 10 min in a or 2 h in b. In a, western blots to analyze the phosphorylation of AKT and ERK induced by the treatments mentioned above. Total AKT, ERK and GAPDH were analyzed as controls. In B, Caspase-3 activity in cell lysates after the different treatments indicated above. Data are expressed as mean ± SEM of n = 3–6. No inhibitor set: ANOVA on ranks p < 0.001 followed by SNK method multiple-comparison post hoc test. LY294002 set: one-way ANOVA p = 0.010 followed by Holm-Sidak method multiple-comparison post hoc test. U0126 set: ANOVA on ranks p = 0.018 followed by Dunn’s method multiple-comparison post hoc test. PD98050 set: one-way ANOVA p < 0.001 followed by Holm-Sidak method multiple-comparison post hoc test. *p < 0.05

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