Table 1 Composition of working solutions for cholesterol detection

Dipotassium hydrogen phosphate (0.1 M)

192

198

Cholesterol oxidase (5U/mL)

6

6

Horse radish peroxidase (50U/mL)

6

6

Sodium taurocholate hydrate (20 mM)

15

15

TritonX-100(1%)

15

15

4-aminoantipyrine (5.5 mM)

45

45

Phenol (280 mM)

15

15

Cholesterol esterase (25U/mL)

6

0

Total volume (μL)

300

300