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Table 1 Composition of working solutions for cholesterol detection

From: Cholesterol-lowing effect of taurine in HepG2 cell

Chemicals TC (μL) FC (μL)
Dipotassium hydrogen phosphate (0.1 M) 192 198
Cholesterol oxidase (5U/mL) 6 6
Horse radish peroxidase (50U/mL) 6 6
Sodium taurocholate hydrate (20 mM) 15 15
TritonX-100(1%) 15 15
4-aminoantipyrine (5.5 mM) 45 45
Phenol (280 mM) 15 15
Cholesterol esterase (25U/mL) 6 0
Total volume (μL) 300 300