Fig. 3

SOD, Cu/Zn-SODandgp91 phox in ox-LDL and Klotho-treated HUVECs. Cellular treatment was the same as described in Fig. 2. a Intracellular SOD activity was determined by the hydroxylamine method. b, c and d Western blotanalysis of Cu/Zn-SOD and gp91 phoxexpression in pre-treated HUVECs. Densitometry of the probed bands from the western blot were analyzed by Image J2x. Values are means ± S.D. (n = 3). Statistical differences are expressed as ## p < 0.01 vs. control; **p < 0.01 vs. ox-LDL