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Table 2 Fatty acid composition of liver phospholipids from control mice and high-fat diet fed mice subjected to hydroxytyrosol (HT) supplementation

From: Hydroxytyrosol prevents reduction in liver activity of Δ-5 and Δ-6 desaturases, oxidative stress, and depletion in long chain polyunsaturated fatty acid content in different tissues of high-fat diet fed mice

  Fatty acid composition (g/100 g FAME)
  Groups
  Control diet (CD) High fat diet (HFD)
  Saline HT Saline HT
Fatty acid (a) (b) (c) (d)
C16:0 31.2 ± 2.6c 28.9 ± 2.1c 43.6 ± 4.2a,b,d 32.4 ± 3.3c
C18:0 4.31 ± 0.6b 3.15 ± 0.2a 4.62 ± 0.6b 4.40 ± 0.4b
C18:1n-9 23.4 ± 1.8 25.6 ± 2.3 22.2 ± 1.6 24.8 ± 2.0
C18:2n-6 (LA) 13.3 ± 1.1 14.5 ± 1.3 11.8 ± 1.0 12.1 ± 1.2
C18:3n-6 1.10 ± 0.1 0.97 ± 0.1 0.95 ± 0.05 0.99 ± 0.05
C18:3n-3 (ALA) 1.19 ± 0.1c 1.23 ± 0.2c 0.89 ± 0.05a,b,d 1.09 ± 0.04c
C18:4n-3 0.25 ± 0.03c 0.27 ± 0.05c 0.09 ± 0.02a,b,d 0.23 ± 0.04c
C20:3n-6 0.27 ± 0.05c 0.29 ± 0.04c 0.15 ± 0.03a,b,d 0.26 ± 0.05c
C20:4n-6 (AA) 10.2 ± 0.9c 11.5 ± 0.7c 5.95 ± 0.4a,b,d 10.4 ± 0.8c
C20:4n-3 0.16 ± 0.04c 0.17 ± 0.05c 0.04 ± 0.02a,b,d 0.14 ± 0.3c
C20:5n-3 (EPA) 1.07 ± 0.1c 1.10 ± 0.1c 0.32 ± 0.04a,b,d 1.05 ± 0.05c
C22:5n-6 (DPAn-6) 0.08 ± 0.01c 0.09 ± 0.02c 0.03 ± 0.01a,b,d 0.08 ± 0.01c
C22:5n-3 (DPAn-3) 0.09 ± 0.02c 0.08 ± 0.01c 0.04 ± 0.01a,b,d 0.08 ± 0.01c
C22:6n-3 (DHA) 4.21 ± 0.2c 4.39 ± 0.3c 1.95 ± 0.1a,b,d 4.12 ± 0.2c
Total SFAs 37.5 ± 3.1c 36.4 ± 2.9c 48.9 ± 4.4a,b,d 38.7 ± 3.5c
Total MUFAs 26.6 ± 2.2 28.5 ± 1.9 24.4 ± 2.4 26.7 ± 2.0
Total PUFAs 35.9 ± 3.1c 35.1 ± 3.6c 26.7 ± 2.3a,b,d 34.6 ± 3.3c
Total LCPUFAs 16.6 ± 1.5c 17.9 ± 1.1c 8.62 ± 0.5a,b,d 16.4 ± 0.9c
Total n-6 LCPUFAs 11.0 ± 0.7c 12.1 ± 0.8c 6.17 ± 0.3a,b,d 10.9 ± 0.8c
Total n-3 LCPUFAs 5.60 ± 0.3c 5.80 ± 0.4c 2.45 ± 0.2a,b,d 5.50 ± 0.5c
n-6/n-3 LCPUFA ratio 1.96 ± 0.1c 2.08 ± 0.05c 2.51 ± 0.2a,b,d 1.98 ± 0.1c
  1. Values are expressed as g fatty acid per 100 g FAME and represent the mean ± SEM for n = 12–14 mice per experimental group. Significant differences between the groups are indicated by the letter identifying each group (one-way ANOVA and the Newman-Keuls´ post-test; p < 0.05). Saturated fatty acids (SFAs) correspond to C12:0, C14:0, C16:0 and C18:0. Monounsaturated fatty acids (MUFAs) correspond to C14:1n-7, C16:1n-7 and C18:1n-9. Polyunsaturated fatty acids (PUFAs) correspond to C18:2n-6, C18:3n-3, C20:4n-6, C20:5n-3, C22:5n-3, and C22:6n-3; n-6 LCPUFAs are C20:4n-6 and C22:5n-3; n-3 LCPUFAs are C20:5n-3, C22:5n-3, and C22:6n-3; n-6/n-3 LCPUFA ratio: C20:4n-6/ (C20:5n-3 + C22:5n-3 + C22:6n-3)