Table 3 Fatty acid composition of erythrocyte phospholipids from control mice and high-fat diet (HFD) fed mice receiving hydroxytyrosol (HT) supplementation

C16:0

34.5 ± 3.1^c

31.9 ± 2.9^c

41.6 ± 3.8^a,b,d

36.6 ± 3.5^c

C18:0

3.62 ± 0.3^c,d

3.45 ± 0.4^c,d

6.58 ± 0.7^a,b

5.58 ± 0.6^a,b

C18:1n-9

24.6 ± 1.9

25.6 ± 2.2

22.9 ± 2.3

24.8 ± 2.5

C18:2n-6 (LA)

9.91 ± 1.2

10.2 ± 0.9

8.66 ± 1.7

9.52 ± 1.5

C18:3n-3 (ALA)

1.21 ± 0.5

1.12 ± 0.3

1.05 ± 0.3

1.05 ± 0.3

C20:4n-6 (AA)

14.6 ± 1.4

15.7 ± 1.6

12.6 ± 1.1

12.4 ± 1.3

C20:5n-3 (EPA)

2.05 ± 0.3^c

2.19 ± 0.4^c

0.75 ± 0.03^a,b,d

1.96 ± 0.2^c

C22:5n-6 (DPAn-6)

0.16 ± 0.04^c

0.19 ± 0.05^c

0.05 ± 0.01^a,b,d

0.12 ± 0.02^c

C22:5n-3 (DPAn-3)

0.78 ± 0.05^c

0.86 ± 0.06^c,d

0.25 ± 0.01^a,b,d

0.70 ± 0.04^b,c

C22:6n-3 (DHA)

4.11 ± 0.3^c

4.32 ± 0.4^c

1.98 ± 0.1^a,b,d

3.98 ± 0.3^c

Total SFAs

38.8 ± 2.9^c

36.5 ± 2.6^c,d

49.7 ± 4.4^a,b

43.5 ± 3.9^b

Total MUFAs

26.4 ± 2.5

27.9 ± 2.4

24.5 ± 2.2

24.8 ± 2.7

Total PUFAs

34.8 ± 3.3^c

35.6 ± 3.6^c

25.8 ± 2.2^a,b

31.7 ± 2.6

Total LCPUFAs

22.9 ± 1.7^c

23.7 ± 2.1^c

15.8 ± 1.1^a,b,d

20.2 ± 1.5^c

Total n-6 LCPUFAs

14.9 ± 1.4

16.0 ± 1.6^c

12.7 ± 0.9^b

12.7 ± 1.4

Total n-3 LCPUFAs

8.00 ± 0.7^c

7.70 ± 0.5^c

3.10 ± 0.2^a,b,d

7.50 ± 0.6^c

n-6/n-3 LCPUFA ratio

1.86 ± 0.2^c

2.07 ± 0.3^c

4.10 ± 0.4^a,b,d

1.69 ± 0.3^c