Table 4 Fatty acid composition of brain phospholipids from control mice and high-fat diet (HFD) fed mice receiving hydroxytyrosol (HT) supplementation

C16:0

35.6 ± 3.1^c

34.3 ± 3.3^c

47.4 ± 4.6^a,b,d

37.3 ± 3.6^c

C18:0

5.95 ± 0.6

5.58 ± 0.4

5.15 ± 0.4

5.75 ± 05

C18:1n-9

19.8 ± 1.6

17.9 ± 1.4

20.4 ± 1.7

19.6 ± 1.8

C18:2n-6 (LA)

3.65 ± 0.2

3.52 ± 0.3

3.12 ± 0.2

3.71 ± 0.4

C18:3n-3 (ALA)

1.30 ± 0.3^c

1.27 ± 0.2^c

0.52 ± 0.05^a,b,d

1.11 ± 0.1^c

C20:4n-6 (AA)

17.1 ± 1.2^c

17.8 ± 1.4^c

11.7 ± 0.7^a,b,d

15.9 ± 1.6^c

C20:5n-3 (EPA)

0.82 ± 0.04^c

0.86 ± 0.03^c,d

0.51 ± 0.02^a,b,d

0.76 ± 0.05^c

C22:5n-6 (DPAn-6)

0.30 ± 0.05^c,d

0.26 ± 0.05^c,d

1.33 ± 0.08^a,b,d

1.10 ± 0.04^a,b,c

C22:5n-3 (DPAn-3)

0.45 ± 0.04^c

0.49 ± 0.05^c,d

0.24 ± 0.02^a,b,d

0.42 ± 0.03^b,d

C22:6n-3 (DHA)

11.5 ± 1.2^c

12.3 ± 1.4^c

7.02 ± 0.6^a,b,d

10.9 ± 1.5^c

Total SFAs

42.5 ± 3.1^c

40.8 ± 2.9^c

52.8 ± 3.8^a,b,d

43.8 ± 3.3^c

Total MUFAs

21.7 ± 1.9

20.6 ± 1.7

22.5 ± 1.5

21.2 ± 2.0

Total PUFAs

35.8 ± 2.8^c

38.6 ± 3.0^c

24.7 ± 2.1^a,b,d

35.0 ± 3.1^c

Total LCPUFAs

30.5 ± 2.3^c

32.6 ± 2.9^c

21.0 ± 1.8^a,b,d

29.5 ± 2.5^c

Total n-6 LCPUFAs

17.6 ± 1.3^c

18.4 ± 1.4^c

13.2 ± 1.0^a,b,d

17.3 ± 1.4^c

Total n-3 LCPUFAs

12.9 ± 1.0^c

14.2 ± 1.1^c

7.80 ± 0.4^a,b,d

12.2 ± 0.8^c

n-6/n-3 LCPUFA ratio

1.36 ± 0.2

1.29 ± 0.2

1.69 ± 0.3

1.41 ± 0.2