Table 2 Real-time quantitative PCR primer sequences
Gene | Forward primer 5′–3′ | Reverse primer 5′–3′ |
|---|---|---|
AMPK | GCCATGCGCAGACTAGTT | AGGATGTATGGCCGATCTTC |
SREBP-1 | GTGAGCCTGACAAGCAATCA | GGTGCCTACAGAGCAAGAGG |
PPARγ | TTTCAAGGGTGCCAGTTT | GAGGCCAGCATCGTGTAG |
PPARα | CCTCAGGGTACCACTACGGAGT | GCCGAATAGTTCGCCGAA |
ACC-l | TACTGAACTACATCTTCTCCC | AAGCAATAAGAACCTGACGA |
FAS | CAAATACAATGGCACCCTGA | TGGCGAAGCCGTAGTTAGTT |
SCD-1 | GAATTCATGCCTGCGCACTTGCTACA | CTCGAGTCAGCCGCTCTTGTGACTCC |
CPT-1 | TTAACAGCAACTACTACGCC | CCAGAAGACGAATAGGTTTGAG |
HSL | GCCACAATGACACAGTCACTGGT | CAGGCAGCGGCCGTAGAAGCA |
ACOX | GGTGGCTGTTAAGAAGAGTG | AAGATGAGTTCCATGACCCA |
c-Jun | ATGACTGCAAAGATGGAAACG | AAGTTGCTGAGGTTGGCG |
c-Fos | TACTACCATTCCCCAGCC | GCTCTACTTTGCCCCTTC |
Fra1 | TCATCTGGAGAGGTGGGTCC | CTGCGGTTCTGACTCACTCG |
TNF-α | AGCCCCCAGTGTGTATCCTT | ACAGTCCAGGTCACTGTCCC |
IL-1β | CTTCAGGCAGGCAGTATCACTC | TTGTTGTTCATCTCGGAGCC |
IL-6 | AGTTGCCTTCTTGGGACTGA | TCCACGATTTCCCAGAGAAC |
β-actin | TTCGTTGCCGGTCCACACCC | GCTTTGCACATGCCGGAGCC |