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Fig. 3 | Lipids in Health and Disease

Fig. 3

From: Inhibition of NAMPT aggravates high fat diet-induced hepatic steatosis in mice through regulating Sirt1/AMPKα/SREBP1 signaling pathway

Fig. 3

Overexpression of NAMPT reverses oleic acid-induced lipid accumulation in HepG2. The cells were transfected with NAMPT plasmid for 24 h and then treated with oleic acid (OA, 0.5 mM) for 24 h. a-b The images (a) and quantitative analysis (b) of NAMPT overexpression in HepG2 cells. c The images of Oil red O staining of HepG2 cells. d The intracellular TG level in HepG2 transfected with NAMPT plasmid or vector under OA stimulation. e Quantitative analysis of the mRNA expressions of lipid synthetic genes including SREBP1, FASN and ACC in HepG2 cells. f The western blot images (left) and quantitative analysis (right) of the protein levels of the lipid synthetic genes including SREBP1, FASN and ACC in HepG2 cells. All results were analyzed based on three independent experiments. The data represent the mean ± SEM. *P < 0.05 and **P < 0.01 versus the control; # P < 0.05 and# # P < 0.01 versus OA

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Lipids in Health and Disease

ISSN: 1476-511X