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Fig. 4 | Lipids in Health and Disease

Fig. 4

From: Inhibition of NAMPT aggravates high fat diet-induced hepatic steatosis in mice through regulating Sirt1/AMPKα/SREBP1 signaling pathway

Fig. 4

Overexpression of NAMPT reverses oleic acid-induced lipid accumulation in Hep1-6. The Hep1-6 cells were transfected with NAMPT plasmid for 24 h and then treated with oleic acid (0.5 mMt) for 24 h. a Quantitative analysis of overexpression of NAMPT by RT-PCR in Hep1-6 cells. b The intracellular TG level in Hep1-6 transfected with NAMPT plasmid or vector under OA stimulation. c Quantitative analysis of the mRNA expressions of lipid synthetic genes including SREBP1, FASN and ACC in Hep1-6 cells. d Western blots images of the expressions of SREBP1 and FASN in Hep1-6 cells. e Quantitative analysis of the expressions of lipid synthetic genes including SREBP1 and FASN by RT-PCR in Hep1-6 cells. All results were analyzed based on three independent experiments. The data represent the mean ± SEM. *P < 0.05 and **P < 0.01 versus the control; # P < 0.05,# # P < 0.01 and ## # P < 0.001 versus OA

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Lipids in Health and Disease

ISSN: 1476-511X