Table 1 Primers for gene amplification in Real-Time qRT-PCR
Gene | Accession no. | Primer sequence (5′-3′) | Length (bp) | Length (bp) | REF |
|---|---|---|---|---|---|
ACTB | NM_001101.3 | F: TCACCCACACTGTGCCCATCTACGA | 25 | 295 | |
R: CAGCGGAACCGCTCATTGCCAATGG | 25 | ||||
PPBP | NM_002704.3 | F: TTGTAGGCAGCAACTCACCC | 20 | 135 | [59] |
R: TGCAAGGCATGAAGTGGTCT | 20 | ||||
DEFA1 /DEFA3 | NM_005217.3 | F: TCCTTGCTGCCATTCTCCTG | 20 | 204 | [60] |
R: TGCACGCTGGTATTCTGCAA | 20 | ||||
HBA2 | NM_000517.4 | F: TCAAGCTCCTAAGCCACTGC | 20 | 162 | [61] |
R: CAGGAGGAACGGCTACCGAG | 20 | ||||
HBB | NM_000518.4 | F: GCAACCTCAAACAGACACCA | 20 | 182 | [62] |
R: CAGCATCAGGAGTGGACAGA | 20 | ||||
IL-1β | NM_000576.2 | F: CCAGCTACGAATCTCCGACC | 20 | 180 | [63] |
R: CTGCCTGCTCTTGGCTAACT | 20 | ||||
CCL3 | NM_002983.2 | F: CTGCAACCAGTTCTCTGCATC | 21 | 145 | [64] |
R: TAGGAAGATGACACCGGGCT | 20 | ||||
CCL4 | NM_002984.3 | F: CCGCCTGCTGCTTTTCTTAC | 20 | 141 | [65] |
R: CACTGGGATCAGCACAGACT | 20 | ||||
SOD2 | NM_001024466.1 | F: TGGAAGCCATCAAACGTGACT | 21 | 173 | [66] |
R: GCCTGTTGTTCCTTGCAGTG | 20 |