Fig. 3
From: DbcAMP regulates adipogenesis in sheep inguinal preadipocytes
Effect of the dose of dbcAMP on the expression of SCD mRNA (a) and HSL mRNA (b) in sheep preadipocyte cultures. Sheep preadipocytes from inguinal adipose tissue were grown in proliferation medium containing 0 nmol/L (as a control), 1 nmol/l, 1 × 102 nmol/l, 1 × 104 nmol/l or 1 × 106 nmol/l dbcAMP for 4 days after cell differentiation induction. Total RNA was harvested and used for first-strand cDNA synthesis. Quantitative real-time PCR analyses were performed to analyze the expression of SCD mRNA and HSL mRNA. The relative expression abundance of a given gene was calculated after normalization to GAPDH mRNA expression. Statistical differences reported are among doses. Values are means ± SEM; n = 3. Values with different letters are significantly different (P < 0.05)