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Fig. 3 | Lipids in Health and Disease

Fig. 3

From: Overexpression of apolipoprotein A-I alleviates endoplasmic reticulum stress in hepatocytes

Fig. 3

ApoA-I overexpression reduces PA-induced lipid accumulation and ER stress in HepG2 cells.HepG2 cells were transfected with pcDNA3.0 alone or the pcDNA3.0/apoA-I plasmid using the FuGENE HD transfection reagent. Twenty-four hours after transfection, the transfected cells were incubated for 24 h in DMEM with 5 mM BSA in the presence or absence of 500 μM PA, and levels of cellular triglycerides (a) as well as free cholesterol (b) were measured. In addition, total cellular proteins were isolated and analysed for GRP78 (c) and CHOP (d) by Western blotting. The results are representative of three independent experiments and are presented as the mean ± SEM. *P < 0.05 versus control. **P < 0.01 versus control. ***P < 0.001 versus control

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