Fig. 3
From: Sake lees extract improves hepatic lipid accumulation in high fat diet-fed mice
The SL extract suppresses lipid accumulation in HepG2 cells. HepG2 cells were treated with fatty acids (100 μM linoleic acid and 100 μM oleic acid) in the presence of or absence of the SL extract (0.1 or 1.0 mg/mL) for 18 h. a Lipid droplets were assessed by Oil Red O staining. The lipid droplets were increased by fatty acids, but the treatment with the SL extract suppressed the increase of intracellular TG. Scale bars indicate upper panel; 100 μm, lower panel; 50 μm. b To quantify lipid accumulation, Oil Red O from the stained cells was eluted by adding 100% isopropanol and the optical density was detected using a spectrophotometer at 490 nm. Data are expressed as the mean ± SEM from three independent experiments. Statistically significant differences between groups were determined using ANOVA; *p < 0.05