Fig. 4
From: 15-oxoeicosatetraenoic acid mediates monocyte adhesion to endothelial cell
15-oxoETE injection enhances monocyte adhesion and E-selectin expression ex vivo. a 15-oxoETE treatment increased THP-1 cell adhesion to the aorta vascular wall of C57 mice. Results were shown as fluorescence in confocal microscopic images. Scale bars = 250 μm. Subsequent images represent the immunohistochemical staining of ICAM-1, VCAM-1, and E-selectin in vascular ECs. Arrows represent E-selectin-positive areas. Scale bars = 50 μm, (n = 3). b 15-oxoETE treatment increased THP-1 cell adhesion to the aorta vascular wall of ApoE−/− mice. Results were shown as fluorescence in confocal microscopic images. Scale bars 100 μm. Subsequent images represent the immunohistochemical staining of ICAM-1, VCAM-1, and E-selectin in vascular ECs. Arrows represent E-selectin-positive areas. Scale bars = 50 μm, (n = 3). c, d Quantification of adhesion on aorta, the result was expressed as areas of monocytes attached to aorta. Data are expressed as mean ± SD. e Quantification of adhesion molecules expression, the fold change is presented as mean ± SD. (**P < 0.01, ***P < 0.001)