Table 1 The primers for real-time RT-PCR
Gene | Primers |
|---|---|
HMG-CoAR | 5'- TACCATGTCAGGGGTACGTC −3' sense |
5'- CAAGCCTAGAGACATAATCATC −3' antisense | |
LDLr | 5'-CCAAATGATGCCACTTCCC −3' sense |
5'- ATCCCATCCCAACACACAC −3' antisense | |
SREBP-2 | 5'- CCCTTCAGTGCAACGGTCATTCAC −3' sense |
5'- TGCCATTGGCCGTTTGTGTC −3' antisense | |
SCAP | 5'- GGCATCAAGTTCTACTCCATTC-3' sense |
5'- CCAGTTGGAATGCTCGGGAC-3' antisense | |
GAPDH | 5'- TGTTGCCATCAACGACCCCTT −3' sense |
5'- CTCCACGACATACTCAGCA −3' antisense |