Table 1 Primers used for quantitative real-time PCR
Genes | Primer sequences | Length |
|---|---|---|
SREBP-1c | Forward 5′-GGAGACATCGCAAACAAGC-3’ | 273 bp |
Reverse 5′-GGTAGACAACAGCCGCATC-3’ | ||
SREBP-2 | Forward 5′-CACCCATACTCAGGCTCG-3’ | 133 bp |
Reverse 5′-GCTTCACAAAGACGCTCAA-3’ | ||
FAS | Forward 5′-ATCGCCTATGGTTGTTG-3’ | 127 bp |
Reverse 5′-TCACGACTGGAGGTTCTA-3’ | ||
ACC | Forward 5′-TATCCCAACTCTTCCCTG-3’ | 116 bp |
Reverse 5′-CCTTCACATAGCCTTTCTC-3’ | ||
HMGCR | Forward 5′-CTTGACGCTCTTGTGGA-3’ | 259 bp |
Reverse 5′-CCCTTTGGGTTACGG-3’ | ||
LXR | Forward 5′-CTCAATGCCTGATGTTTCTCC-3’ | 154 bp |
Reverse 5′-TGACTCCAACCCTATCCCTAA-3’ |