Table 1 Specific primers used for real-time PCR analysis
Gene (NCBI Genbank) | Humans | Rats |
|---|---|---|
HSL (NM_005357) | Sense: AACTGCCAGCTGCCTTAAAA Antisense: TTCCCTCACGGGAGATATTG | Sense: CCTCAAAGTCAAACCCTCCA Antisense: GTGCGTAAATCCATGCTGTG |
ATGL (NM_020376) | Sense: TCCTCGGCGTCTACTACGTC Antisense: CTCAATGAACTTGGCACCAG | Sense: CAACGCCACTCACATCTACG Antisense: AGCAGGCAGGGTCTTCAGTA |
CGI-58 (NM_016006) | Sense: GTGCCCTAGGATTGGACAAA Antisense: GGCTCTGATCCAAACTGGAA | Sense: TGTGTCCCCTGCACTTACAA Antisense: AAAATTCAGGGCCCAAAGTC |
Perilipin 1 (NM_002666) | Sense: CCCAGGAGTGACAGGAATTG Antisense: CTCGCTCCTCAAGCTTCAAC | Sense: ATCTCCTGCCACCAGACAAG Antisense: GATCCACATGGCCAGAGAGT |
Leptin (NM_013076) | Sense: ACAGAAAGTCACCGGTTTGG Antisense: GTGAAGAAGATCCCGGAGGT | Sense: CCTGGTGACAATGGTCTTGA Antisense: AGCTGCAAGGTCCAAGAAGA |
Adiponectin (NM_144744) | Sense: ATG ACCAGGAAACCACGACT Antisense: CACC GATGTCTCCCTTAGGA | Sense: CATCTCCTGGGTCACCCTTA Antisense: ACCCAAGGAAACTTGTGCAG |
Visfatin (NM_177928) | Sense: AAAATCCAGGAAGCCAAAGA Antisense: TCCTCTGGGAATGACAAAGC | Sense:TTTCCTCGTATTTCACCTTCC Antisense: AAGCCGAGTTCAACATCCTG |
18S (HQ387008) | Sense: CCTGCGGCTTAATTTGACTC Antisense: ATGCCAGAGTCTCGTTCGTT | --------------------------------------------- --------------------------------------------- |
GAPDH (NM_017008) | ---------------------------------------------- ---------------------------------------------- | Sense: AGACAGCCGCATCTTCTTGT Antisense: CTTGCCGTGGGTAGAGTCAT |