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Fig. 3 | Lipids in Health and Disease

Fig. 3

From: The purification of reduced β2-glycoprotein I showed its native activity in vitro

Fig. 3

LC/MS-based peptide characterization method development for reduced β2GPI. The theoretical MWs of the target peptide (TDASDVKPC) in different charge and alkylation form was described (a). The digestion product after reduction and reduction plus alkylation were compared (b & c). b shows the reduced peptide form of MH2+, while c shows the alkylated peptide form of MH2+. Especially in Fig. c, alkylated peptide can be characterized only in the reduction plus alkylation sample, which implies that if the cysteine is blocked with GSH, then it will not be alkylated

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