Skip to main content
Fig. 4 | Lipids in Health and Disease

Fig. 4

From: The purification of reduced β2-glycoprotein I showed its native activity in vitro

Fig. 4

The final LC/MS characterization of the reduced and GSH-blocked β2GPI. a Reduced β2GPI blocked by GSH showed different MWs non-reduced SDS-PAGE conditions. b The LC/MS ion peak of GSH blocked reduced β2GPI. c The LC/MS ion peak of IAA blocked β2GPI. The sample conditions and the profile comparison on SDS-PAGE was visualized (a). The samples in lane 7 and lane 5 were digested and analysed by LC/MS. The reduced and GSH-blocked β2GPI sample shows only the reduced peptide form of MH2+ (b), while the reduced and alkylated β2GPI sample shows only the alkylated peptide form of MH2+. These results proved that our reduced and GSH-blocked β2GPI was produced in the right form

Back to article page