Fig. 7

Reduced β2GPI inhibited LPS-mediated inflammation in mice. The effect of reduced β2GPI on the levels of IL-6 (a), TNF-α (b), IL-12P70 (c), IFN-γ (d), IL-10 (e) and MCP-1 (f) in mice serum upon LPS stimulation. Purified plasma β2GPI (2 μM) or reduced β2GPI with 1 nM LPS were preincubated for 15 min at 37 °C before injection into mice at 1 μg/g body weight (gbw) with E. coli LPS (Sigma-Aldrich) in sterile saline or the same volume of saline with TRX-1 (1.75 μM) + DTT (35 μM) + GSH (80 mM) through the tail vein. At 6 h post injection, mice were euthanized, and approximately 1 ml of blood was collected by cardiac puncture. Serum was collected and the levels of cytokines (IL-6, TNF-α, MCP-1, IFN-γ, IL-10, IL-12P70) were quantified. Reduced β2GPI significantly reduced the levels of IL-6, TNF-α, MCP-1, and IFN-γ. No difference was observed (n = 5) between β2GPI only or GSH and LPS. For all panels, *P < 0.05 and **P < 0.001